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human embryonic lung hel299 cells  (ATCC)


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    Structured Review

    ATCC human embryonic lung hel299 cells
    (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) <t>HEL299</t> cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.
    Human Embryonic Lung Hel299 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 309 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung hel299 cells/product/ATCC
    Average 95 stars, based on 309 article reviews
    human embryonic lung hel299 cells - by Bioz Stars, 2026-05
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    Images

    1) Product Images from "Dual Host-Virus Arms Races Shape an Essential Housekeeping Protein"

    Article Title: Dual Host-Virus Arms Races Shape an Essential Housekeeping Protein

    Journal: PLoS Biology

    doi: 10.1371/journal.pbio.1001571

    (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) HEL299 cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.
    Figure Legend Snippet: (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) HEL299 cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.

    Techniques Used: Virus, Binding Assay, Residue, Selection, Stable Transfection, Plasmid Preparation, Infection, Expressing, Labeling, Standard Deviation



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    human embryonic lung hel299 cells  (ATCC)
    95
    ATCC human embryonic lung hel299 cells
    (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) <t>HEL299</t> cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.
    Human Embryonic Lung Hel299 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung hel299 cells/product/ATCC
    Average 95 stars, based on 1 article reviews
    human embryonic lung hel299 cells - by Bioz Stars, 2026-05
    95/100 stars
    		  Buy from Supplier
    human embryonic lung cells (hel299)  (BioResource International Inc)
    90
    BioResource International Inc human embryonic lung cells (hel299)
    (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) <t>HEL299</t> cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.
    Human Embryonic Lung Cells (Hel299), supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/human embryonic lung cells (hel299)/product/BioResource International Inc
    Average 90 stars, based on 1 article reviews
    human embryonic lung cells (hel299) - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier
    hel299 human embryonic lung cells  (Korean Cell Line Bank)
    90
    Korean Cell Line Bank hel299 human embryonic lung cells
    (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) <t>HEL299</t> cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.
    Hel299 Human Embryonic Lung Cells, supplied by Korean Cell Line Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hel299 human embryonic lung cells/product/Korean Cell Line Bank
    Average 90 stars, based on 1 article reviews
    hel299 human embryonic lung cells - by Bioz Stars, 2026-05
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) HEL299 cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.

    Journal: PLoS Biology

    Article Title: Dual Host-Virus Arms Races Shape an Essential Housekeeping Protein

    doi: 10.1371/journal.pbio.1001571

    Figure Lengend Snippet: (A) The apical domain of human TfR1 is shown in green, in a co-crystal structure with the Machupo GP1 shown in grey (PDB 3KAS) . The βII-1–βII-2 species-specific virus binding motif (residues 204–212) is highlighted in yellow. The side chains of residue positions identified as evolving under positive selection are shown in red. A human SNP (L212V; rs41301381) has been reported at position 212, shown in blue. (B) MDCK, (C) HEK293, or (D) HEL299 cells were stably transduced to express either 212L TfR1 or 212V TfR1 (or an empty vector) and then infected with various amounts of Machupo pseudovirus. Virus entry is scored by percentage of cells that become GFP positive (+). The error bars in the HEL299 experiment are large due to difficulty in sorting these cells. Nonetheless, this pattern of relative entry between the different TFR1 alleles expressed in HEL299 cells was observed in four independent experiments (not shown). (B, C, D, right-hand panels) Relative cell-surface expression of human TfR1 variants in each cell line was measured on live cells with a fluorescently labeled α-FLAG antibody. In all experiments, three replicates were performed and error bars indicate one standard deviation.

    Article Snippet: Human embryonic kidney 293T cells (ATCC CRL-11268), HEK293 cells (ATCC CRL-1573), human embryonic lung HEL299 cells (ATCC CCL-137), and canine kidney MDCK.2 cells (ATCC CRL-2936) were all maintained in Dulbecco modified Eagle's medium (Cellgro) supplemented with 10% fetal bovine serum (Gibco), 100 units ml −1 penicillin, 100 μg ml −1 streptomycin, and 2 mM L-glutamine (Cellgro).

    Techniques: Virus, Binding Assay, Residue, Selection, Stable Transfection, Plasmid Preparation, Infection, Expressing, Labeling, Standard Deviation